Detection by PCR of Anaplasma in Danish Roe deer

Sigurdur Skarphedinsson 1; B. Lyholm 1, P.M. Jensen 2

1. Department of Clinical Microbiology, Odense University Hospital, Denmark
2. Department of Ecology, The Royal Veterinary and Agricultural University, Denmark

Ehrlichiosis, caused by Anaplasma (formerly Ehrlichia) phagocytophilia is an important emerging tick-borne infection. The role of Roe deer (Capriolus capriolus) in the epidemiology of ehrlichiosis in Denmark was investigated. Blood samples were collected during the hunting season (15/5- 15/7 ’02 and 1/10- 15/1 ’03) from 22 Danish forest districts. Blood samples from 237 Roe deer were tested for Anaplasma phagocytophilia genogroup 16S rRNA gene sequences by PCR. Overall a mean of 47% Roe deer were found Anaplasma positive in PCR. Marked seasonal variation in PCR positive Roe deer was found between the two hunting periods (71% vs. 26%). These findings confirm that roe deer are commonly infected with Anaplasma and provide evidence that roe deer are a major reservoir of Anaplasma in Denmark.

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