Genetic diversity over short geographic distances and no host specificity among Bartonella grahamii infecting woodland rodents of central Sweden

Ehrenborg, Christian (1); Handley, Scott (2); Ellis, Barbara (3); Mills, James (3); Holmberg, Martin (1)

(1) Dept of Medical Sciences, Section for Infectious Diseases, Uppsala University Hospital;
(2) Molecular Microbiology and Microbial Pathogenesis,Washington University, St.Louis, Missouri, USA;
(3) Centers for Disease Control and Prevention, Atlanta, USA

Bartonella grahamii was the dominant species isolated during a previously published field study in central Sweden in 1999. Genotypic diversity among 31 Bartonella grahamii isolates was assessed by comparison of DNA sequences derived from gltA and ftsZ gene fragments and by infrequent restriction site PCR (IRS-PCR). The ftsZ sequences revealed two variants which corresponded to two clusters appearing in the IRS-PCR analysis. One cluster consisted of isolates from animals captured from two localities not divided by any apparent natural boundary, and the other cluster mainly contained isolates from one location that was separated from the others by a bay. The B. grahamii variants were not related to specific host species which corroborates the findings of low host specificity by others. Natural rather than species boundaries seem to restrict gene flow.

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